Heart rate variability (HRV) describes the variations between consecutive inter-beat-intervals or IBIs. Both sympathetic and parasympathetic branches of the ANS are involved in the regulation of heart rate (HR). Sympathetic nervous system (SNS) activity increases HR and decreases HRV, whereas parasympathetic nervous system (PNS) activity decreases HR and increases HRV Berntson et al. 1997. The control of the autonomic output involves several interconnected areas of central nervous system, which form the so-called central autonomic network. In addition to this central control, arterial baroreceptor reflex as well as respiration are known to induce quick changes in heart rate. The baroreflex is based on baroreceptors which are located on the walls of some large vessels and can sense the stretching of vessel walls caused by pressure increase. Both sympathetic and parasympathetic activity are influenced by baroreceptor stimulation trough a specific baroreflex arc (Fig. 1).

Blood pressure regulation and baroreflex
Figure 1: Functioning of the barorelfex arc.

Typically, the most conspicuous oscillatory component of HRV is the respiratory sinus arrhythmia (RSA), where the vagus nerve stimulation is being cut-off during inhalation, and thus, HR increases during inhalation and decreases during exhalation (see video below). This high frequency (HF) component of HRV is thus centered at respiratory frequency and is considered to range from 0.15 to 0.4 Hz. Another conspicuous component of HRV is the low frequency (LF) component ranging from 0.04 to 0.15 Hz. The HF component is mediated almost solely by the PNS activity, whereas the LF component is mediated by both SNS and PNS activities and is also affected by baroreflex activity Task Force 1996, Berntson et al. 1997 . The origin of the LF oscillations is however considered to be dominated by SNS and the normalized power of the LF component could be used to assess sympathetic efferent activity Pagani et al. 1997, Furlan et al. 2000. The fluctuations below 0.04 Hz, on the other hand, have not been studied as much as the higher frequencies. These frequencies are commonly divided into very low frequency (VLF, 0.003-0.04 Hz) and ultra low frequency (ULF, 0-0.003 Hz) bands, but in case of short-term recordings the ULF band is generally omitted Task Force 1996. These lowest frequency rhythms are characteristic for HRV signals and have been related to, e.g., humoral factors such as the thermoregulatory processes and renin-angiotensin system Berntson et al. 1997.

Heart rate variability (HRV) is a commonly used tool when trying to assess the functioning of cardiac autonomic regulation. It has been used in multitude of studies, related to cardiovascular research and different human wellbeing applications, as an indirect tool to evaluate the functioning and balance of the autonomic nervous system (ANS).

Overview of Respiratory Sinus Arrhythmia (RSA)

One of the main clinical scenarios where HRV has been found valuable include the risk stratification of sudden cardiac death after acute myocardial infarction Task Force 1996, Acharya et al. 2006, Laitio et al. 2007, Pradhapan et al. 2014. In addition, decreased HRV is generally accepted to provide an early warning sign of diabetic cardiovascular autonomic neuropathy Task Force 1996, Acharya et al. 2006, the most significant decrease in HRV being found within the first 5-10 years of diabetes Vinik et al. 2013, Tarvainen et al. 2014. Besides these two main clinical scenarios, HRV has been studied with relation to several cardiovascular diseases, renal failure, physical exercise, occupational and psychosocial stress, gender, age, drugs, alcohol, smoking and sleep van Ravenswaaij-Arts et al. 1993, Malik et al. 1993, Task Force 1996, Pumprla et al. 2002, Achten et al. 2003, Acharya et al. 2006.


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QRS detection

Electrocardiogram (ECG)
Figure 2: Electrophysiology of the heart (redrawn from Malmivuo & Plonsey 1995). The different waveforms for each of the specialized cells found in the heart are shown. The latency shown approximates that normally found in the healthy heart.

The aim in HRV analysis is to examine the sinus rhythm modulated by the autonomic nervous system. Therefore, one should technically detect the occurrence times of the SA-node action potentials, which initiate every heart beat. In practical applications, this is not however possible. Instead, an the electrocardiogram (ECG) is recorded by placing two or more electrodes on skin contact and heart beats are detected from the ECG. The nearest observable activity in the ECG compared to SA-node firing is the P-wave resulting from atrial depolarization (see Fig. 2). The signal-to-noise ratio of the P-wave is, however, clearly lower than that of the strong QRS complex which results primarily from ventricular depolarization. Therefore, the heart beat period is commonly evaluated as the time difference between the easily detectable QRS complexes. A typical QRS detector consists of a preprocessing part followed by a decision rule. Several different QRS detectors have been proposed within last decadesThakor et al. 1983, Pahlm et al. 1985, Pan & Tompkins 1985, Hamilton et al. 1984, Friesen et al. 1990

The accuracy of the R-wave occurrence time estimates is often required to be 1–2 ms, and thus, the sampling frequency of ECG should be at least 500–1000 Hz Task Force 1996. If the sampling frequency of ECG is less than 500 Hz, the errors in R-wave occurrence times can cause critical distortion to HRV analysis results, especially to spectrum estimates Merri et al. 1990. The distortion of the spectrum is even bigger if the overall variability in heart rate is small Pinna et al. 1994. The estimation accuracy can however be improved by interpolating the QRS complex e.g. by using a cubic spline interpolation Daskalov et al. 1997 or some model based approach Bragge et al. 2005.

Pulse detection

Photoplethysmography (PPG) is a technique for monitoring blood volume changes in the micro vascular bed of tissue. Shortly after the QRS complex appears in the ECG, the ventricular systole generates a pulse wave which leads to a rapid increase in blood pressure and blood volume, this change is seen by the steep rise in the pulse wave (see Fig. 3). The subsequent decline corresponds to cardiac diastole and may contain a secondary peak, the so-called dicrotic notch, which is attributed to the closure of the aortic valve. Pulse-to-pulse interval (PP-interval) is defined as a time interval between the rising part of two consecutive pulse waves.

Pulse wave detection from PPG signal
Figure 3: Normal PPG end ECG signal and definitions of pulse transmit time (PTT) and pulse to pulse interval (PP).

Depending on the pulse wave velocity and the vascular path from the heart, there is a delay between each QRS complex and the onset of its corresponding pulse wave. The delay is called pulse transit time (PTT) and is negatively correlated with blood pressure, arterial stiffness, and age. Physiological variability in PTT causes deviation between the PP-intervals and the RR-intervals. Since PP and RR intervals are not equal, it is always better to use the term pulse rate variability (PRV) rather than HRV when PPG measurement is used. Usability and accuracy of PRV as an estimate of HRV has been widely studied. A good review on the topic can be found from Schafer et al 2013, with the following concluding remarks:

  • PRV as an estimate of HRV has been proved to be sufficiently accurate only for healthy (and mostly younger) subjects at rest.
  • Moderate physical or mental stress tends to diminish agreement between PRV and HRV to an extent that is or is not acceptable.
  • Physically more active states, such as walking or physical exercising, the agreement between PRV and HRV often becomes insufficient, mostly due to motion artifacts.

Beat detection algorithms in Kubios HRV software

In case ECG data is imported into Kubios HRV software, the R-wave time instants are automatically detected by applying a built-in QRS detection algorithm. This in-house developed detection algorithm is based on the Pan–Tompkins algorithmPan & Tompkins 1985. The detector consists of a preprocessing part followed by decision rules.The preprocessing part includes bandpass filtering of the ECG (to reduce power line noise, baseline wander and other noise components), squaring of the data samples (to highlight peaks) and moving average filtering (to smooth close-by peaks). The decision rules include amplitude threshold and comparison to expected value between adjacent R-waves. Both of these rules are adjusted adaptively every time a new R-wave is acceptably detected. Before R-wave time instant extraction, the R-wave is interpolated at 2000 Hz to improve the time resolution of detection. The up-sampling can significantly improve the time resolution of R-wave detection when the sampling rate of the ECG is low.

Pulse interval accuracy
Figure 4: Bland-Altman plot for the difference between PP intervals and RR intervals extracted from resting measurements (left axes). The errors in standard HRV parameters when computed from PRV compared to HRV time series (right axes); where red lines show the median, the box shows the 25-75 percentiles, and the whiskers show the most extreme error values.

Pulse wave detector of Kubios HRV software is based on the matched filtering approach. Firstly, maximum of 1st derivative representing the steepest part of the pulse wave is used for initial pulse location estimation. Secondly, a template for the pulse wave (and matched filter) is constructed using the initial pulses. Decision of the final pulse wave locations are defined by comparing the filtered signal against varying threshold and comparing normalized error between the template and the PPG signal. Allowed normalized error between template and pulse wave under inspection can be adjusted in software preferences. That is, the smaller the acceptance threshold percent is the more similar the pulse wave have to be with the template in order to be accepted. The accuracy of the pulse wave detection algorithm is shown in Fig. 4. The left panel showing the Bland-Altman plot illustrating the agreement between detected PP intervals and corresponding RR intervals during a resting measurement. The right panel shows error percentages of commonly used HRV parameters estimated from PP interval compared to RR interval time series. Used dataset contains 20 healthy volunteers with wide age scale (20 to 50 years). The error between the RR and PP intervals was -0.01±5.16 ms (mean ± SD). This ±5 ms error in heart beat detection produces approximately ±10 % maximum errors to the HRV parameters.

Inter-beat interval (IBI) time series

Derivation of heart rate variability (HRV) time series from ECG signal
Figure 5: Derivation of two HRV time series from ECG: the interval tachogram (middle panel) and interpolated RR interval series (bottom panel).

After the beat occurrence times (i.e. QRS complex or pulse wave fiducial points) have been detected, an inter-beat interval (IBI) time series can be derived. Instead of IBI, we will here use the term RR interval, which refers to time interval between successive ECG R-wave occurrence times. The n‘th RR interval is obtained as the difference between the R-wave occurrence times {\rm RR}_n = t_n-t_{n-1}. In some context, normal-to-normal (NN) may also be used when referring to these intervals indicating strictly intervals between successive QRS complexes resulting from SA-node depolarization Task Force 1996. In practice when analysing normal sinus rhythm, the NN and RR intervals are the same, and thus, the term RR is preferred here.

The time series constructed from all available RR intervals is not equidistantly sampled, but has to be presented as a function of time, i.e. as values (t_n,{\rm RR}_n). This fact has to be taken into account before frequency-domain analysis. In general, three different approaches have been used to get around this issue Task Force 1996. The simplest approach that have been adopted e.g. in Baselli et al. 1987 is to assume equidistant sampling and calculate the spectrum directly from the RR interval tachogram (RR intervals as a function of beat number), see Fig. 5 middle panel. This assumption can however cause distortion into the spectrum Mateo et al. 2000. This distortion becomes substantial when the variability is large in comparison to the mean RR interval length. Furthermore, the spectrum can not be considered to be a function of frequency, but rather a function of cycles per beat Task Force 1996, see Fig. 5 bottom panel. One choice for the interpolation method is the cubic spline interpolation Mateo et al. 2000. After interpolation, regular spectrum estimation methods can be applied. The third general approach is to apply methodology, which are designed for analysing non-equidistantly sampled data. Such a method is for example the Lomb-Scargle periodogram, which computes the periodogram spectrum estimate for non-equidistantly sampled data van Dongen et al. 1999.


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Careful preprocessing of RR interval data is very important before analysing or interpreting HRV data. Any artefact in the RR interval time series may significantly interfere the analysis of these signals. The artefacts within HRV signals can be divided into technical and physiological artefacts. The technical artifacts include missing, extra or misaligned QRS complex detections. These artefacts may be due to measurement noise or due to incompetence of the detection algorithm. Physiological artefacts include ectopic beats and arrhythmic events. In order to avoid the interference of such artefacts, the ECG recording and the corresponding event series should always be manually checked for artefacts and only artefact-free sections should be included in the analysis Task Force 1996. Alternatively, if the amount of artefact-free data is insufficient, proper interpolation methods can be used to reduce these artefacts, see e.g. Lippman et al. 1993, Lippman et al. 1994, Mateo et al. 2003.

In addition to artefacts, slow changes in mean HR during the recording can have undesirable effects on certain HRV analysis parameters. Such slow nonstationarities are characteristic for HRV signals and should be considered before the analysis. The origins of nonstationarities in HRV are discussed e.g. in Berntson et al. 1997. The slow nonstationary trends can be easily removed from the HRV time series before analysis. Detrending can be performed using first order Litvack et al. 1995, Mitov et al. 1998 or higher order polynomial Porges et al. 1990, Mitov et al. 1998 models. In addition, Kubios HRV software includes an advanced detrending procedure originally presented in Tarvainen et al. 2002. This approach is based on smoothness priors regularization and is described in detail below.

Artefact correction methods

Kubios HRV software provides excellent tools for handling artefacts. First, the R-wave detections can be corrected manually when
ECG data is available, which provides true beat intervals but is reasonable only when when data includes a limited number of misdetections. Secondly, the software includes two artefact correction methods: 1) Threshold based correction algorithm, and 2) Automatic correction algorithm. Details of these correction methods are given below.

Threshold based artefact correction algorithm

The threshold based artefact correction algorithm compares every RR interval value against a local average interval. The local average is obtained by median filtering the RR interval time series, and thus, the local average is not affected by single outliers in RR interval time series. If an RR interval differs from the locale average more than a specified threshold value, the interval is identified as an artefact and is marked for correction. The threshold value can be selected from:

  • Very low: 0.45 sec (threshold in seconds)
  • Low: 0.35 sec
  • Medium: 0.25 sec
  • Strong: 0.15 sec
  • Very strong: 0.05 sec
  • Custom, for setting a custom threshold in seconds

For example, the “Medium” correction level will identify all RR intervals that are larger/smaller than 0.25 seconds compared to the local average. The correction is made by replacing the identified artefacts with interpolated values using a cubic spline interpolation. It should be noted, that these threshold are adjusted with mean heart rate. That is, thresholds shown above are for HR of 60 beats/min, but for higher HR the thresholds are smaller (because the variability is expected to decrease when HR increases) and vice versa for lower heart rates.

Because the artefacts are identified by simple thresholding and normal HRV level is highly individual, the correction level should be adjusted individually as follows. First, identify if there are any artefacts in your data that should be corrected. If there are artefacts, then select the lowest possible correction level, which identifies the artefacts but does not identify too many normal RR intervals as artefacts.

Automatic artefact correction algorithm

In automatic correction algorithm artefacts are detected from dRR series, which is a time series consisting of differences between successive RR intervals. The dRR series provides a robust way to separate ectopic and misplaced beats from the normal sinus rhythm. To separate ectopic and normal beats, time varying threshold (Th) is used. To ensure adaptation to different HRV levels, the threshold is estimated from the time-varying distribution of dRR series. For each beat, quartile deviation of the 90 surrounding beats is calculated and multiplied by factor 5.2. Beats within this range cover 99.95% of all beats if RR series is normally distributed. However, RR interval series is not often normally distributed, and thus, also some of the normal beats exceed the threshold. Therefore, decision algorithm is needed to detect artefact beats.

Table 1: Performance of the automatic artefact correction algorithm in detecting normal sinus rhythm beats, and simulated missed, extra and misaligned (with three different levels of displacement) beats.
Sinus beats 61 757 99.963%
Missed beats 610 100%
Extra beats 610 100%
Misaligned beats (q=2) 610 58 ms 53.9%
Misaligned beats (q=4) 610 119 ms 99.3%
Misaligned beats (q=6) 610 238 ms 100%

Ectopic beats form negative-positive-negative (NPN) or positive-negative-positive (PNP) patterns to the dRR series. Similarly long beats form positive-negative (PN) and short beats negative-positive (NP) patterns to the dRR series. Only dRR segments containing these patterns are classified as artefact beats. Missed or extra beats are detected by comparing current RR value with median of the surrounding 10 RR interval values (medRR). A missed beat is detected if current RR interval (RR(i)) satisfies condition

(1)   \begin{equation*} \left|\frac{RR(i)}{2}-medRR(i)\right|<2 Th \end{equation*}

and an extra beat is detected if two successive RR intervals (RR(i) and RR(i+1)) satisfies condition

(2)   \begin{equation*} \left|RR(i)+RR(i+1)-medRR(i)\right|<2 Th. \end{equation*}

Correction of detected artefacts: Detected ectopic beats are corrected by replacing corrupted RR times by interpolated RR values. Similarly too long and short beats are corrected by interpolating new values to the RR time series. Missed beats are corrected by adding new R-wave occurrence time and extra beats are simply corrected by removing extra R-wave detection and recalculating RR interval series.

Detrending of RR interval data

The theory behind the smoothness priors detrending methods inluded in Kubios HRV software is described shortly here. For more details, see Tarvainen et al. 2002.

Let z\in\R^N denote the RR interval time series which can be considered to consist of two components

(3)   \begin{equation*} z = z_{\rm{stat}}+z_{\rm{trend}} \end{equation*}

where z_{\rm{stat}} is the nearly stationary RR interval series of interest, z_{\rm{trend}} is the low frequency aperiodic trend component, and N is the number of RR intervals. Suppose that the trend component can be modeled with a linear observation model as

(4)   \begin{equation*} z_{\rm{trend}} = H\theta + e \end{equation*}

where H\in\R^{N\times p} is the observation matrix, \theta\in\R^p are the regression parameters, and e is the observation error. The task is then to estimate the parameters by some fitting procedure so that \hat{z}_{\rm{trend}} = H\hat{\theta} can be used as the estimate of the trend. The properties of the estimate depend strongly on the properties of the basis vectors (columns of the matrix H) in the fitting. A widely used method for the solution of the estimate \hat{\theta} is the least squares method. However, a more general approach for the estimation of \hat{\theta} is used here. That is, the so-called regularized least squares solution

(5)   \begin{equation*} \hat{\theta}_\lambda = \arg \min_\theta \left\{ \Vert z-H\theta \Vert^2 + \lambda^2\Vert D_2 (H\theta)\Vert^2 \right\} \end{equation*}

where \lambda is the regularization parameter and D_2 indicates the discrete approximation of 2nd order derivative. This is a modification of the ordinary least squares solution, dragging the solution to the direction in which the norm \Vert D_2(H\theta)\Vert gets smaller. In this way, prior information about the predicted trend H\theta can be implemented to the estimation. The solution of (5) can be written in the form

(6)   \begin{equation*} \hat{\theta}_\lambda = (H^TH + \lambda^2H^TD_2^TD_2H)^{-1} H^T z \end{equation*}

and the estimate for the trend which is to be removed can be written as

(7)   \begin{equation*} \hat{z}_{\rm{trend}} = H\hat{\theta}_\lambda. \end{equation*}

The selection of the observation matrix H can be implemented according to some known properties of the data z. However, here we use a trivial choice of identity matrix H = I\in\R^{N\times N}. In this case, the regularization part of (5) simply draws the solution towards a curve for which the 2nd order difference is zero, i.e. towards first order linear curve. With these specific choices, the detrended nearly stationary RR series can be written as

(8)   \begin{equation*} \hat{z}_{\rm{stat}} = z- H\hat{\theta}_\lambda = (I-(I+\lambda^2D_2^TD_2)^{-1}) z. \end{equation*}

This detrending method works like a time-varying highpass filter, where the cutoff frequency can be adjusted by changing the smoothing parameter \lambda. In Kubios HRV Premium software, the cutoff frequency related to the given smoothing parameter is presented. The smoothing parameter is by default selected to remove only VLF frequency components from the RR time series.


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In this section, the analysis methods available in Kubios HRV software are introduced. The presented methods are mainly based on the guidelines given in Task Force 1996. The presentation of the methods is divided into four categories, i.e. time-domain, frequency-domain, nonlinear, and time-varying methods. The methods are summarized in Table 1.

Time-domain methods

The time-domain methods are derived from the beat-to-beat RR interval values in time domain. Let the RR interval time series include N successive beat intervals, i.e. {\rm RR}=({\rm RR}_1, {\rm RR}_2, \ldots, {\rm RR}_N). The mean RR interval (\overline{\rm RR}) and the mean heart rate (\overline{\rm HR}) are then defined as

(9)   \begin{equation*} \overline{\rm RR} = \frac{1}{N}\sum_{n=1}^N {\rm RR}_n,\quad \overline{\rm HR} &=& \frac{60}{\overline{\rm RR}} \end{equation*}

where {\rm RR}_n denotes the value of n‘th RR interval. Several HRV parameters that measure the variability within the RR time intervals in time-domain exist. The standard deviation of RR intervals (SDNN) is defined as

(10)   \begin{equation*} {\rm SDNN} = \sqrt{\frac{1}{N-1}\sum_{n=1}^N ({\rm RR}_n-\overline{\rm RR})^2} \end{equation*}

SDNN reflects the overall (both short-term and long-term) variation within the RR interval time series, whereas the standard deviation of successive RR interval differences (SDSD) given by

(11)   \begin{equation*} {\rm SDSD} = \sqrt{E\{\Delta{\rm RR}_n^2\} - E\{\Delta{\rm RR}_n\}^2} \end{equation*}

is a measure of short-term (beat-by-beat) variability. For stationary RR series E\{\Delta{\rm RR}_n\} = E\{{\rm RR}_{n+1}\}-E\{{\rm RR}_n\} = 0 and SDSD equals the root mean square of successive differences (RMSSD) given by

(12)   \begin{equation*} {\rm RMSSD} = \sqrt{\frac{1}{N-1}\sum_{n=1}^{N-1} ({\rm RR}_{n+1} - {\rm RR}_n)^2}. \end{equation*}

Another measure calculated from successive RR interval differences is the NN50 which is the number of successive intervals differing more than 50 ms or the corresponding relative amount

(13)   \begin{equation*} {\rm pNN50} = \frac{\rm NN50}{N-1} \times 100\% . \end{equation*}

Computation of geometric measures of heart rate variability (HRV) (triangular index and TINN)
Figure 6: Computation of geometric measures of HRV: A) triangular index (HRVi) and triangular interpolation of RR intervals (TINN), and B) Baevsky’s stress index.

In addition to the above statistical measures, there are some geometric measures that are calculated from the RR interval histogram. The HRV triangular index is obtained as the integral of the histogram (i.e. total number of RR intervals) divided by the height of the histogram which depends on the selected bin width. In order to obtain comparable results, a bin width of 1/128 seconds is recommended Task Force 1996. Another geometric measure is the TINN which is the baseline width of the RR histogram evaluated through triangular interpolation, see Figure 6A.

The Baevsky’s stress index (SI) is computed according to the formula Baevsky 2009

(14)   \begin{equation*} {\rm SI} = \frac{{\rm AMo}\times 100\%}{2{\rm Mo\times MxDMn}} \end{equation*}

where AMo is the so-called mode amplitude presented in percent, Mo is the mode (the most frequent RR interval) and MxDMn is the variation scope reflecting degree of RR interval variability (see Figure 6B). The mode Mo is simply taken as the median of the RR intervals. The AMo is obtained as the height of the normalised RR interval histogram (bin width 50 msec) and MxDMn as the difference between longest and shortest RR interval values. In order to make SI less sensitive to slow changes in mean heart rate (which would increase the MxDMn and lower AMo), the very low frequency trend is removed from the RR interval time series by using the smoothness priors method Tarvainen et al 2002. In addition, the square root of SI is taken to transform the tailed distribution of SI values towards normal distribution. The square root transformation also makes the SNS index described later less sensitive to extreme SI values.

Frequency-domain methods

In the frequency-domain methods, a power spectrum density (PSD) estimate is calculated for the RR interval series. The regular spectrum estimators implicitly assume equidistant sampling and, thus, the RR interval series is converted to equidistantly sampled series by interpolation methods prior to spectrum estimation. In Kubios HRV software a cubic spline interpolation method is used. In HRV analysis, the spectrum is generally estimated using either a Fast Fourier transformation (FFT) based methods or parametric autoregressive (AR) modeling based methods. For details on these methods see, e.g., Marple 1987. The advantage of FFT based methods is the simplicity of implementation, while the AR spectrum yields improved resolution especially for short samples. Another property of AR spectrum that has made it popular in HRV analysis is that it can be factorized into separate spectral components.

In Kubios HRV software, the HRV spectrum is calculated with FFT based Welch’s periodogram method and with the AR method. Spectrum factorization in AR method is optional. In the Welch’s periodogram method the HRV sample is divided into overlapping segments. The spectrum is then obtained by averaging the spectra of these segments, which decreases the variance of the FFT spectrum.

Kubios HRV software includes also the Lomb-Scargle periodogram van Dongen et al. 1999, which differs from the Welch’s periodogram in the sense that it does not assume equidistant sampling and is thus computed directly from the non-interpolated RR interval time series. The variance of the Lomb-Scargle periodogram is decreased by smoothing the periodogram using MA-filering (the window width of the MA-filter can be adjusted in software preferences).

Heart rate variability (HRV) spectrum estimation (LF and HF power)
Figure 7: HRV spectrum estimates using FFT based Welch’s periodogram method (left) and autoregressive (AR) modelling based spectrum estimation method.

The generalized frequency bands in case of short-term HRV recordings are the very low frequency (VLF, 0–0.04 Hz), low frequency (LF, 0.04–0.15 Hz), and high frequency (HF, 0.15–0.4 Hz). The frequency-domain measures extracted from a spectrum estimate for each frequency band include absolute and relative powers of VLF, LF and HF bands; LF and HF band powers in normalized units; the LF/HF power ratio; and peak frequencies for each band (see Table 1). In the case of FFT spectrum, absolute power values for each frequency band are obtained by simply integrating the spectrum over the band limits. In the case of AR spectrum, on the other hand, if factorization is enabled distinct spectral components emerge for each frequency band with a proper selection of the model order and the absolute power values are obtained directly as the powers of these components. If factorization is disabled the AR spectrum powers are calculated as for the FFT spectrum. The band powers in relative and normalized units are obtained from the absolute values as described in Table 1.

Nonlinear methods

Considering the complex control systems of the heart it is reasonable to assume that nonlinear mechanisms are involved in heart rate requlation. The nonlinear properties of HRV have been analysed using measures such as Poincaré plot Brennan et al. 2001, Carrasco et al. 2001, approximate and sample entropy Richman & Moorman 2000, Fusheng et al. 2001, detrended fluctuation analysis Peng et al. 1995, Penzel et al. 2003, correlation dimension Guzzetti et al 1996, Henry et al. 2001, and recurrence plots Webber et al. 1994, Trulla et al. 1996, Zbilut et al. 2002. During the last years, the number of studies utilizing such methods have increased substantially. The downside of these methods is still, however, the difficulty of physiological interpretation of the results.

Poincaré plot
Poincare plot analysis of heart rate variability (HRV) data
Figure 8: Poincaré plot analysis with the ellipse fitting procedure. SD1 and SD2 are the standard deviations perpendicular to and along the the line-of-identity {\rm RR}_n = {\rm RR}_{n+1}, respectively.

One commonly used nonlinear method that is simple to interpret is the so-called Poincaré plot. It is a graphical representation of the correlation between successive RR intervals, i.e. plot of {\rm RR}_{n+1} as a function of {\rm RR}_n. The shape of the plot is essential and a common approach to parameterize the shape is to fit an ellipse to the plot as shown in Fig. 8. The ellipse is oriented according to the line-of-identity ({\rm RR}_n = {\rm RR}_{n+1}). The standard deviation of the points perpendicular to the line-of-identity denoted by SD1 describes short-term variability which is mainly caused by RSA. It can be shown that SD1 is related to the time-domain measure SDSD according toBrennan et al. 2001

(15)   \begin{equation*} \rm SD1^2 = \frac{1}{2} SDSD^2 \end{equation*}

where SDSD is defined as {\rm SDSD}=\sqrt{E[\Delta {\rm RR}_n^2] - \overline{\Delta {\rm RR}_n}^2}, which is equal to RMSSD for stationary time series. The standard deviation along the line-of-identity denoted by SD2, on the other hand, describes long-term variability and has been shown to be related to time-domain measures SDNN and SDSD by Brennan et al. 2001

(16)   \begin{equation*} \rm SD2^2 = 2\, SDNN^2 - \frac{1}{2} SDSD^2. \end{equation*}

The standard Poincaré plot can be considered to be of the first order. The second order plot would be a three dimensional plot of values ({\rm RR}_n,\RR_{n+1},{\rm RR}_{n+2}). In addition, the lag can be bigger than 1, e.g., the plot ({\rm RR}_n,{\rm RR}_{n+2}).

Approximate entropy

Approximate entropy (ApEn) measures the complexity or irregularity of the signal Richman & Moorman 2000, Fusheng et al. 2001. Large values of ApEn indicate high irregularity and smaller values of ApEn more regular signal. The ApEn is computed as follows.

First, a set of length m vectors u_j is formed

(17)   \begin{equation*} u_j = ({\rm RR}_j,{\rm RR}_{j+1},\ldots,{\rm RR}_{j+m-1}),\quad j= 1,2,\ldots N-m+1 \end{equation*}

where m is called the embedding dimension and N is the number of measured RR intervals. The distance between these vectors is defined as the maximum absolute difference between the corresponding elements, i.e.

(18)   \begin{equation*} d(u_j,u_k) = \max\left\{\vert {\rm RR}_{j+l} - {\rm RR}_{k+l}\vert \,\bigl\vert\, l =  0,\ldots,m-1\right\}. \end{equation*}

Next, for each u_j the relative number of vectors u_k for which d(u_j,u_k)\le r is calculated. This index is denoted with C_j^m(r) and can be written in the form

(19)   \begin{equation*} C_j^m(r) = \frac{{\rm nbr\, of\,}\left\{ u_k\,\bigl\vert\, d(u_j,u_k)\le r \right\}}{N-m+1}\quad\forall\, k. \end{equation*}

Due to the normalization, the value of C_j^m(r) is always smaller or equal to 1. Note that the value is, however, at least 1/(N-m+1) since u_j is also included in the count. Then, take the natural logarithm of each C_j^m(r) and average over j to yield

(20)   \begin{equation*} \Phi^m(r) = \frac{1}{N-m+1}\sum_{j=1}^{N-m+1} \ln{C_j^m(r)}. \end{equation*}

Finally, the approximate entropy is obtained as

(21)   \begin{equation*} {\rm ApEn}(m,r,N) = \Phi^m(r)-\Phi^{m+1}(r). \end{equation*}

Thus, the value of the estimate ApEn depends on three parameters, the length m of the vectors u_j, the tolerance value r, and the data length N. In Kubios HRV software the default value of m is set to be m=2. The length N of the data also affects ApEn. When N is increased the ApEn approaches its asymptotic value. The tolerance r has a strong effect on ApEn and it should be selected as a fraction of the standard deviation of the RR interval data (SDNN). This selection enables the comparison of RR data from different subjects. A common selection for r is r = 0.2 {\rm SDNN}, which is also the default value in Kubios HRV software.

Sample entropy

Sample entropy (SampEn) is similar to ApEn, but there are two important differences in its calculation Richman & Moorman 2000, Lake et al. 2002. For ApEn, in the calculation of the number of vectors u_k for which d(u_j,u_k)\le r also the vector u_j itself is included. This ensures that C_j^m(r) is always larger than 0 and the logarithm can be applied, but at the same time it causes bias to ApEn. In sample entropy the self-comparison of u_j is eliminated by calculating C_j^m(r) as

(22)   \begin{equation*} C_j^m(r) = \frac{{\rm nbr\, of\,}\left\{ u_k\,\bigl\vert\, d(u_j,u_k)\le r \right\}}{N-m}\quad\forall\, k\neq j. \end{equation*}

Now the value of C_j^m(r) will be between 0 and 1. Next, the values of C_j^m(r) are averaged to yield

(23)   \begin{equation*} C^m(r) = \frac{1}{N-m+1}\sum_{j=1}^{N-m+1} C_j^m(r) \end{equation*}

and the sample entropy is obtained as

(24)   \begin{equation*} {\rm SampEn}(m,r,N) = \ln{(C^m(r)/C^{m+1}(r))}. \end{equation*}

The default values set for the embedding dimension m and for the tolerance parameter r in Kubios HRV software are the same as those for the ApEn computation. Both ApEn and SampEn are estimates for the negative natural logarithm of the conditional probability that a data of length N, having repeated itself within a tolerance r for m points, will also repeat itself for m+1 points. SampEn was designed to reduce the bias of ApEn and has a closer agreement with the theory for data with known probabilistic content Lake et al. 2002.

Multiscale entropy (MSE)

Multiscale entropy (MSE) is an extension of SampEn in the sense that it incorporates two procedures Costa et al. 2005

  1. A coarse-graining process is applied to the RR interval time series. Multiple coarse-grained time series are constructed for the time series by averaging the data points within non-overlapping windows of increasing length \tau, where \tau represents the scale factor and is selected to range between \tau=1,2,\ldots,20. The length of each coarse-grained time series is N/\tau, where N is the number of RR intervals in the data. For scale \tau=1, the coarse-grained time series is simply the original beat-to-beat RR interval time series.
  2. SampEn is calculated for each coarse-grained time series. SampEn as a function of the scale factor produces the MSE. MSE for scale factor \tau=1 returns standard SampEn (computed from the original data points).
Detrended fluctuation analysis

Detrended fluctuation analysis (DFA) measures the correlation within the signal. The correlation is extracted for different time scales as follows Peng et al. 1995. First, the RR interval time series is integrated

Detrended fluctuation analysis (DFA) of heart rate variability (HRV) data
Figure 9: Detrended fluctuation analysis. A double log plot of the index F(n) as a function of segment length n. \alpha_1 and \alpha_2 are the short term and long term fluctuation slopes, respectively.

(25)   \begin{equation*} y(k) = \sum_{j=1}^k ({\rm RR}_j - \overline{\rm RR}),\quad k = 1,\ldots,N \end{equation*}

where \overline{\rm RR} is the average RR interval. Next, the integrated series is divided into segments of equal length n. Within each segment, a least squares line is fitted into the data. Let y_n(k) denote these regression lines. Next the integrated series y(k) is detrended by subtracting the local trend within each segment and the root-mean-square fluctuation of this integrated and detrended time series is calculated by

(26)   \begin{equation*} F(n) = \sqrt{\frac{1}{N}\sum_{k=1}^N (y(k)-y_n(k))^2}. \end{equation*}

This computation is repeated over different segment lengths to yield the index F(n) as a function of segment length n. Typically F(n) increases with segment length. A linear relationship on a double log graph indicates presence of fractal scaling and the fluctuations can be characterized by scaling exponent \alpha (the slope of the regression line relating \log{F(n)} to \log{n}. Different values of \alpha indicate the following

  • \alpha = 1.5: Brown noise (integral of white noise)
  • 1 < \alpha < 1.5: Different kinds of noise
  • \alpha = 1: 1/f noise
  • 0.5 < \alpha < 1:Large values are likely to be followed by large value and vice versa
  • \alpha = 0.5: White noise
  • 0 < \alpha < 0.5: Large value is likely to be followed by small value and vice versa

Typically, in DFA the correlations are divided into short-term and long-term fluctuations. In Kubios HRV software, the short-term fluctuations are characterized by the slope \alpha_1 obtained from the (\log{n},\log{F(n)}) graph within range 4\le n \le 12 (default values). Correspondingly, the slope \alpha_2 obtained by default from the range 13\le n \le 64 characterizes long-term fluctuations, see Fig. 9.

Correlation dimension

Another method for measuring the complexity or strangeness of the time series is the correlation dimension which was proposed in Grassberger et al. 1983. The correlation dimension is expected to give information on the minimum number of dynamic variables needed to model the underlying system and it can be obtained as follows.

Similarly as in the calculation of approximate and sample entropies, form length m vectors u_j

(27)   \begin{equation*} u_j = ({\rm RR}_j,{\rm RR}_{j+1},\ldots,{\rm RR}_{j+m-1}),\quad j= 1,2,\ldots, N-m+1 \end{equation*}

and calculate the number of vectors u_k for which d(u_j,u_k)\le r, that is

(28)   \begin{equation*} C_j^m(r) = \frac{{\rm nbr\, of\,}\left\{ u_k\,\bigl\vert\, d(u_j,u_k)\le r \right\}}{N-m+1}\quad\forall\, k \end{equation*}

where the distance function d(u_j,u_k) is now defined as

    (29)   \begin{equation*} d(u_j,u_k) = \sqrt{\sum_{l=1}^m \left( u_j(l)-u_k(l)\right)^2}. \end{equation*}

Correlation dimension analysis of heart rate variability (HRV) data
Figure 10: Approximation of the correlation dimension D_2 from the (\log r, \log C^m(r)) plot.

Next, an average of the term C_j^m(r) is taken

(30)   \begin{equation*} C^m(r) = \frac{1}{N-m+1}\sum_{j=1}^{N-m+1} C_j^m(r) \end{equation*}

which is the so-called correlation integral. The correlation dimension
D_2 is defined as the limit value

(31)   \begin{equation*} D_2(m) = \lim_{r\rightarrow 0}\lim_{N\rightarrow \infty} \frac{\log{C^m(r)}}{\log{r}}. \end{equation*}

In practice this limit value is approximated by the slope of the regression curve (\log{r},\log{C^m(r)}) Henry et al. 2001. The slope is calculated from the linear part of the log-log plot, see Fig. 10. The slope of the regression curves tend to saturate on the finite value of D_2 when m is increased. In the software, a default value of m=10 was selected for the embedding.

Recurrence plot analysis

Yet another approach, included in the software, for analyzing the
complexity of the time series is the so-called recurrence plot (RP) analysis. In this approach, vectors

(32)   \begin{equation*} u_j = ({\rm RR}_j,{\rm RR}_{j+\tau},\ldots,{\rm RR}_{j+(m-1)\tau}),\quad j = 1,2,\ldots,N-(m-1)\tau \end{equation*}

where m is the embedding dimension and \tau the embedding lag. The vectors u_j then represent the {\rm RR} interval time series as a trajectory in m dimensional space. A recurrence plot is a symmetrical [N-(m-1)\tau]\times [N-(m-1)\tau] matrix of zeros and ones. The element in the j‘th row and k‘th column of the RP matrix, i.e. RP(j,k), is 1 if the point u_j on the trajectory is close to point u_k. That is

(33)   \begin{equation*} {\rm RP}(j,k) = \left\{\begin{array}{ll} 1, & d(u_j-u_k)\le r \\ 0, & {\rm otherwise} \end{array}\right. \end{equation*}

where d(u_j,u_k) is the Euclidean distance given in (29) and r is a fixed threshold. The structure of the RP matrix usually shows short line segments of ones parallel to the main diagonal. The lengths of these diagonal lines describe the duration of which the two points are close to each other. An example RP for HRV time series is presented in Fig. 11. Methods for quantifying recurrence plots were proposed in Webber et al. 1994. The methods included in this software are introduced below.

Recurrence plot (RP) analysis of heart rate variability (HRV) data
Figure 11: Recurrence plot matrix for HRV time series (black = 1 and white = 0).

In the software the following selections were made. The embedding dimension and lag were selected to be m=10 (default value) and \tau=1 (fixed), respectively. The threshold distance r was selected to be
\sqrt{m}\, {\rm SD} (default value), where SD is the standard deviation of the RR time series. The selection are similar to those made in
Dabire et al 1998.

The first quantitative measure of RP is the recurrence rate (REC) which is simply the ratio of ones and zeros in the RP matrix. The number of elements in the RP matrix for \tau=1 is equal to N-m+1 and the recurrence rate is simply given as

(34)   \begin{equation*} {\rm REC} = \frac{1}{(N-m+1)^2}\sum_{j,k = 1}^{N-m+1} {\rm RP}(j,k). \end{equation*}

The recurrence rate can also be calculated separately for each diagonal parallel to the line-of-identity (main diagonal). The trend of REC as a function of the time distance between these diagonals and the line-of-identity describes the fading of the recurrences for points further away. %This trend can be obtained by linear regression.

The rest of the RP measures consider the lengths of the diagonal lines. A threshold l_{\rm min} = 2 is used for excluding the diagonal lines formed by tangential motion of the trajectory. The maximum line length is denoted l_{\rm max} and its inverse, the divergence,

(35)   \begin{equation*} {\rm DIV} = \frac{1}{l_{\rm max}} \end{equation*}

has been shown to correlate with the largest positive Lyapunov exponent Trulla et al. 1996. The average diagonal line length, on the other hand, is obtained as

(36)   \begin{equation*} l_{\rm mean} = \frac{\sum_{l=l_{\min}}^{l_{\rm max}} l N_{l}}{\sum_{l=l_{\rm min}}^{l_{\rm max}} N_{l}} \end{equation*}

where N_{l} is the number of length l lines. The determinism of the time series is measured by the variable

(37)   \begin{equation*} {\rm DET} = \frac{\sum_{l=l_{\rm min}}^{l_{\rm max}} lN_{l}}{\sum_{j,k=1}^{N-m+1} {\rm RP}(j,k)}. \end{equation*}

Finally, the Shannon information entropy of the line length distribution is defined as

(38)   \begin{equation*} {\rm ShanEn} = -\sum_{l=l_{\rm min}}^{l_{\rm max}} n_{l} \ln n_{l} \end{equation*}

where n_{l} is the number of length l lines divided by the total number of lines, that is

(39)   \begin{equation*} n_{l} = \frac{N_{l}}{\sum_{l'=l_{\rm min}}^{l_{\rm max}} N_{l'}}. \end{equation*}

PNS and SNS indexes

Parasympathetic nervous system (PNS) activity (vagal stimulation) is known to decrease heart rate and increase HRV. The sympathetic nervous system (SNS) activity having the opposite effect on HR and HRV, i.e. it increases HR and decreases HRV. Therefore, HR is lowest and HRV is highest when we are in rest and fully recovered. During stressful situations when sympathetic nervous activity is increased, out HR is higher and HRV is smaller.

The PNS index in Kubios HRV software is computed based on Mean RR (ms), RMSSD (ms) and SD1 (%). Cardiac vagal activity is known to increase mean RR interval (i.e. decrease HR), thus Mean RR is a natural choice for the PNS index computation. In addition to mean heart rate, cardiac vagal activity affects HRV by regulating the magnitude of the respiratory sinus arrhythmia (RSA) component. The RSA is observed as quick changes in RR interval linked to respiration, i.e. decrease in RR during inhalation and increase in RR during exhalation . In time-domain these quick beat-to-beat changes in RR interval can be best captured by the RMSSD parameter, which is why this parameter is the second input for the PNS index computation. In frequency-domain, the RSA component is typically observed as a peak within the high frequency (HF) band of the RR interval spectrum. This HF component is centered at the respiratory rate and its power is known to correlate strongly with the RMSSD. The power of the HF component in normalized units provides an alternative index for parasympathetic activity, and similarly, the low frequency (LF) component in normalized units (or the LF/HF ratio) has been widely used as an index of sympathetic activity or sympathovagal balance Task Force 1996. The problem with the HF component is that in case of spontaneous breathing, especially when the natural breathing rate of the subject is low (below 0.15 Hz or 9 breaths/min), the RSA component is partially, or even completely, overlaping with the LF component. Since Poincare plot index SD1 is known to be linked to RMSSD Brennan et al. 2001 and the ratio SD2/SD1 correlates with LF/HF ratio, the normalized SD1 value is used in Kubios HRV as the third input parameter for the PNS index computation.

The SNS index in Kubios HRV software is computed based on Mean HR (bpm), Baevsky’s stress index and SD2 (%). The mean HR is an evident choice, since the increase in HR is known to be linked to increased cardiac sympathetic activation. The Baevsky’s stress index is a rather widely used index of cardiovascular system stress and is strongly linked to sympathetic nervous activity. Finally, the normalized Poincare plot parameter SD2 provides a robust index of sympathovagal balance and is thus used as the third input parameter for SNS index computation.

During exercise cardiac vagal activity withdrawal followed by increased sympathetic activation takes place causing HR to increase as a function of exercise intensity. A curvilinear decrease in HRV shown both in time-domain and frequency-domain variables is observed during exercise and the minimum level of variability is typically reached at moderate to high intensity. RMSSD associated to parasympathetic activity typically reaches the minimum level already at moderate intensity (120-140 bpm, or 50-60% VO2max). The normalized LF power (or similarly SD2 (%)) typically increases during low-moderate intensity exercise and decresaes during higher intensity exercise, while normalized HF power (or SD1 (%)) shows the opposite exercise related response. In addition, a slight increase in HRV close to maximum intensities have been observed in some studies, but this might originate from non-neural mechanisms such as direct mechanical effects of respiration on the SA node. Scott et al. 2017

In conclusion, the PNS index is computed from Mean RR (ms), RMSSD (ms) and SD1 (%); and SNS index is from Mean HR (bpm), Baevsky’s stress index and SD2 (%). Each parameter value is first compared to their normal population values as presented in Nunan et al. 2010. The normal values for the SD1 and SD2 are derived based on their dependency on the time-domain variables RMSSD and SDNN as described in Brennan et al. 2001. The normal values for the Baevsky’s stress index are taken from Baevsky 2009. The parameter values are then scaled with the standard deviations of normal population and finally a proprietary weighting is applied to obtain robust and reliable PNS and SNS index values. The known links between exercise intensity, heart rate and HRV (as shortly described above) have also been taken into account in the optimization of the weighs adopted in PNS and SNS index computations.

Computation of parasympathetic (PNS) and sympathetic nervous system (SNS) indexes for heart rate variability (HRV) data
Figure 12: PNS and SNS index computations.

The interpretation of the PNS and SNS indexes is straightforward. A PNS (or SNS) index value of zero means that the parameters reflecting parasympathetic (or sympathetic) activity are on average equal to the normal population average. Correspondingly, non-zero PNS index values describe how many SDs below (negative values) or above (positive values) the normal population average the parameter values are. Please note that the normal values presented in Nunan et al. 2010 are extracted from resting HRV measurements. Thus in rest, the PNS and SNS indexes are typically (with 95% of population) within -2…+2, i.e. within ±2SD of the normal population distribution (see Fig. 12). But during stress or during high intensity exercise much lower PNS index and much higher SNS index values can be observed.

Time-varying analysis

Time-varying analysis enables us to examine the changes occurring in RR interval time series as a function of time. Time-varying trends for time-domain and nonlinear HRV parameters can be obtained by applying a moving window over the RR data. The window should be long enough to provide accurate HRV analysis, but too long window tends to over-smooth the changes in RR data. A good choice for the analysis window is 5-10 minutes when analyzing longer measurementsTask Force 1996. The window shift only affects on the time grid in which the time-varying analysis results are provided. In Kubios HRV software, both the window width and the shift can be defined by the user. The trends of the frequency-domain HRV parameters are computed based on a time-varying spectrum estimate. In Kubios HRV software, the time-varying spectrum is estimated either by using the short-time Fourier transformation (also known as spectrogram) method or by using a time-varying AR spectrum estimate. In the time-varying AR method, AR coefficients are estimated iteratively by using a Kalman smoother algorithm. The adaptation of the Kalman smoother algorithm can be adjusted, which affects on the resolution of the spectrum. For details on the Kalman smoother spectrum estimation see Tarvainen et al. 2006. An example of time-varying analysis of a 48-hour HRV recording is shown in Fig. 13.

Stress and recovery evaluation based on heart rate variability (HRV) analysis
Figure 13: An example of time-varying analysis of 48-hour HRV recording; showing time-varying spectrum and EDR (top), and PNS and SNS index time trends (bottom).

The time-varying methods of Kubios HRV software include the trends of the time-domain measures \overline{\rm RR}, SDNN, \overline{\rm HR}, Min HR, Max HR, RMSSD, NNxx, pNNxx, HRV triangular index and TINN. For frequency-domain measures the trends are obtained for VLF, LF, and HF peak frequencies; VLF, LF, and HF band powers (both in ms^2 and log-transformed); relative LF and HF band powers (both in \% and in n.u. units); and LF/HF ratio. Also the ECG derived respiration (EDR) is presented under frequency-domain parameters when ECG data is available. In addition, trends are calculated for the nonlinear measures Poincar{\e’} plot SD1, SD2 and SD2/SD1 ratio; approximate and sample entropies (ApEn and SampEn); detrended fluctuation analysis (DFA) slopes \alpha_1 and \alpha_2.

Summary of HRV parameters

A summary of the most commonly used HRV parameters is given in the table below under Time-domain, Frequency-domain and Nonlinear categories. Under the Overview category, description of additional variables available in Kubios HRV software are summarized.

Table 2: Summary of the HRV parameters calculated by Kubios HRV software.
PNS index Parasympathetic nervous system activity compared to normal resting values
SNS index Sympathetic nervous system activity compared to normal resting values
HR zones [%] Time spent in Maximum, Hard, Moderate, Light, Very light, and Inactive HR zones
Stress zones [%] Time spent in Very high, High, Elevated, Normal, and Low stress levels
RMSSD zones [%] Time spent in Very low, Low, Lowered, Normal, and High RMSSD levels
Energy exp. [kcal] Energy expenditure is divided into Basal Metabolic Rate (BMR), diet-induced thermogenesis (DIT) and activity related energy expenditure. BMR is esitmated with Mifflin-St Jeor formula and energy expenditure (EE) using Keytel’s modelMifflin et al. 1990, Keytel et al. 2005
[ms] The mean of RR intervals
STD RR (SDNN) [ms] Standard deviation of RR intervals
[beats/min] The mean heart rate
Min & Max HR [beats/min] Minimum and maximum HR computed using N beat moving average (default value: N=5)
RMSSD [ms] Square root of the mean squared differences between successive RR intervals
NNxx [beats] Number of successive RR interval pairs that differ more than xx ms (default value: xx=50)
pNNxx [%] NNxx divided by the total number of RR intervals
HRV triangular index The integral of the RR interval histogram divided by the height of the histogram
TINN [ms] Baseline width of the RR interval histogramTask Force 1996
Stress index Square root of Baevsky’s stress indexBaevsky 2009
SDANN [ms] Standard deviation of the averages of RR intervals in 5-min segmentsTask Force 1996
SDNNI [ms] Mean of the standard deviations of RR intervals in 5-min segmentsTask Force 1996
Spectrum Welch’s (or Lomb-Scargle) periodogram and AR spectrum estimates
Peak frequency [Hz] VLF, LF, and HF band peak frequencies
Absolute power [ms2] Absolute powers of VLF, LF, and HF bands
Absolute power [log] Natural logarithm transformed values of absolute powers of VLF, LF, and HF bands
Relative power [%] Relative powers of VLF, LF, and HF bands:
VLF [%] = VLF [ms2] / total power [ms2] x 100%
LF [%] = LF [ms2] / total power [ms2] x 100%
HF [%] = HF [ms2] / total power [ms2] x 100%
Normalized power [n.u.] Powers of LF and HF bands in normalised units:
LF [n.u.] = LF [ms2] / (total power [ms2] – VLF [ms2]) x 100%
HF [n.u.] = HF [ms2] / (total power [ms2] – VLF [ms2]) x 100%
LF/HF Ratio between LF and HF band powers
EDR [Hz] ECG derived respiration (available only if ECG data is used for HRV analysis)
SD1 [ms] In Poincaré plot, the standard deviation perpendicular to the line-of-identity Brennan et al. 2001, Carrasco et al. 2001
SD2 [ms] In Poincaré plot, the standard deviation along the line-of-identity
SD2/SD1 Ratio between SD2 and SD1
ApEn Approximate entropyRichman & Moorman 2000, Fusheng et al. 2001
SampEn Sample entropyRichman & Moorman 2000
DFA, α1 In detrended fluctuation analysis, short term fluctuation slopePeng et al. 1995, Penzel et al. 2003
DFA, α2 In detrended fluctuation analysis, long term fluctuation slope
D2 Correlation dimensionGuzzetti et al. 1996, Henry et al. 2001
RPA Recurrence plot analysisWebber et al. 1994, Dabire et al. 1998, Zbilut et al. 2002
  Lmean [beats] Mean line length
  Lmax [beats] Maximum line length
  REC [%] Recurrence rate
  DET [%] Determinism
  ShanEn Shannon entropy
MSE Multiscale entropy for scale factor values τ=1,2,…,20 Costa et al. 2005


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